2026.05.21

Functionality for Skin Radiance and Dullness (1): In Vitro Evaluation of Efficacy

Loss of skin translucency is believed to result from reduced light transmittance caused by the carbonylation of keratin, the primary structural protein of the stratum corneum. Carbonylation induces changes in the fibrous structure of keratin, thereby decreasing the transmission of light through the skin.

The inhibitory effect of pineapple-derived ceramides on the carbonylation of stratum corneum proteins has been investigated using stratum corneum samples collected by the tape-stripping method, and the results of these studies have been reported.

The stratum corneum can be treated with hypochlorous acid to induce carbonylation artificially.

The carbonylated stratum corneum is visualized using a fluorescent dye and quantified through image analysis.

Using the stratum corneum carbonylated by hypochlorous acid treatment as the 0% inhibition control, the carbonylation inhibition rate of samples treated with Pineapple Ceramide in combination with hypochlorous acid was calculated.

In vitro is a Latin term meaning “in glass.” In research, it refers to experiments in which human or animal tissues (cells) are cultured to evaluate the efficacy and safety of test substances.

Functional Effects on Skin Translucency and Dullness (1): In Vitro Evaluation

Source：FOOD STYLE21

Supervisor

President: Born in Gunma Prefecture in 1965, he completed his doctoral studies at the Graduate School of Agricultural Science at Tokyo University of Agriculture (PhD), and served as a special research fellow at the Japan Society for the Promotion of Science, a part-time lecturer at Tokyo University of Agriculture, and an associate professor and professor at Takasaki University of Health and Welfare before becoming a professor at Tokyo University of Agriculture in April 2012. He is a professor at the same graduate school, and will be the president of Tokyo University of Agriculture in April 2021. He will be the chairman of Tokyo University of Agriculture in July 2023.

School he attended
April 1984 - March 1988 Graduated from the Department of Forestry (Forestry Science Course), Faculty of Agriculture, Tokyo University of Agriculture

Degrees he received
Tokyo University of Agriculture - Doctor of Forestry

Career history within the university
April 2012 - Ongoing Professor, Department of Forest Science, Faculty of Regional Environmental Science, Tokyo University of Agriculture
April 2016 - Ongoing Director, Food and Agriculture Museum, Tokyo University of Agriculture (Other organizations)
April 2021 - Ongoing President, Tokyo University of Agriculture

Academic societies and committees he has been affiliated with
April 1985 - Ongoing Japan Wood Research Society
June 1988 - Ongoing Japanese Mushroom Society
April 1995 - Ongoing Japanese Society of Inflammatory and Regenerative Medicine
April 1995 - Ongoing Japanese Society of Bioscience, Biotechnology, and Agricultural Chemistry
May 1995 - Ongoing Applied Pharmacology Research Society